Deoxycytidylate deaminase activity of simian virus 40-infected cell cultures.
نویسندگان
چکیده
ney cell extracts with dCTP also exhibit as much activity as do those from vacciniaand Simian Virus 40 (SV40)-infected cultures, respectively. Extracts prepared with dCTP from vacciniaor herpes simplexinfected mouse embryo and mouse kidney cultures have no more than 1.7 times the deoxycytidylate deaminase activity of uninfected cell extracts; however, thymidine kinase activity of virus-infected cell extracts is greatly increased over that of un infected cell extracts. Either productive-polyoma virus infection or abortive-SV40 infection of mouse kidney cells result in stimulations of both deoxycytidylate deaminase and thymidine kinase activities. These activities remain elevated in SV40-transformed mouse kidney cell cultures. Puromycin, but not 1-0-D-arabinofuranosylcytosine, inhibits the induction of deoxycytidylate deaminase in SV40-infected mouse kidney cultures.
منابع مشابه
Deoxycytidylate Deaminase Activity of Simian Virus 40-infected Cell Cultures1
ney cell extracts with dCTP also exhibit as much activity as do those from vacciniaand Simian Virus 40 (SV40)-infected cultures, respectively. Extracts prepared with dCTP from vacciniaor herpes simplexinfected mouse embryo and mouse kidney cultures have no more than 1.7 times the deoxycytidylate deaminase activity of uninfected cell extracts; however, thymidine kinase activity of virus-infected...
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The activities ofdCMP deaminase andDNA polymerase I increased twofold and fivefold in BHK-21/C13 cells after infection by the virus of herpes simplex. The increases were greatly diminished, and under certain conditions prevented, by inclusion of actinomycin D or cycloheximide in the cell-virus system during the infective cycle. ThedCMP deaminase purified from infected cells harvested 8h after i...
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عنوان ژورنال:
- Cancer research
دوره 27 10 شماره
صفحات -
تاریخ انتشار 1967